Spatially resolved omics technologies are providing radically new frameworks for interrogating biological systems. This has tremendous implications for cancer research in areas such as intratumoral heterogeneity, tumor microenvironment, cell-cell signaling, spatial phenotyping, and metastasis. In this Cell Press webinar, Marianna Rapsominiki (IBM Research, Zurich) and Itai Yanai (New York University) discuss how advances in spatial biology can help unravel some of the deepest questions in cancer.