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Quantitative analysis of proteins using advanced mass spectrometry (MS) is a powerful tool to gain insight into fundamental biological processes. While molecular characterization of RNA at the single cell level has had a major impact on all areas of biology over the last 5 years, doing so at the protein level is still in its infancy.

Low amounts of protein, combined with the high number of samples required for analysis, presents great challenges for single cell MS research.

In this webcast, the speaker will describe how a 384-well workflow, using standard off-the-shelf components, allows characterization of single cells to a protein depth of well over 1,000 proteins per cell.  The webcast will introduce the latest generation of HRAM Thermo Scientific™ Tribrid™ MS instrumentation with FAIMS technology for single cell-level sensitivity. When combined with new Thermo Scientific™ TMTPro reagents and optimized chromatography, researchers can analyse  >100 single cells per day per instrument. An in-house developed computational pipeline, SCeptre, helps derive biological meaning from the data.

These advances in single cell MS sample preparation and analysis provide a robust way to collect cellular proteomes from mammalian cells, paving the way for large-scale single cell MS experiments at unprecedented depth.