In this webinar, we will explore the critical role of controlling polysaccharide molecular weight in the production of conjugate vaccines. The session will delve into the use of high-shear processing, specifically the Dyhydromatics® ShearJet® HL60, to achieve precise degradation of polysaccharides.

We will discuss how variations in concentration and the number of passes through the high-shear processor impact the final product, characterized using size-exclusion chromatography with multi-angle light scattering (SEC-MALS).

This technique ensures a more uniform product with reduced molecular weight and polydispersity, enhancing reproducibility and product quality.

Key Learning Objectives:

1. Understand the Importance of Polysaccharide Molecular Weight Control: Learn why controlling the molecular weight of polysaccharides is crucial for the consistency and potency of conjugate vaccines
2. Explore High-Shear Processing Techniques: Gain insights into the mechanical degradation of polysaccharides using the Dyhydromatics® ShearJet® HL60 and how it compares to chemical hydrolysis
3. Analyze the Impact of Processing Parameters: Discover how different concentrations and the number of passes through the high-shear processor affect the molecular weight and polydispersity of polysaccharides
4. Characterize Polysaccharides with SEC-MALS: Understand the principles and advantages of using SEC-MALS for accurate characterization of polysaccharide molecular weight and polydispersity

• Biotech and Vaccine Industry Professionals: Individuals involved in the research, development, and production of conjugate vaccines
• Analytical Chemists and Biochemists: Professionals interested in the characterization and analysis of polysaccharides and other polymers
• Process Engineers: Those looking to optimize high-shear processing techniques for improved product quality and consistency
• Quality Control Specialists: Individuals responsible for ensuring the reproducibility and quality of vaccine products