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Protein interactions orchestrate a plethora of cellular processes and are thus essential to understand cellular function. However, their analysis is challenging – especially in the native context. In this webcast, Dr. Panagiotis Kastritis will explore the feasibility of deriving vital structural insights for protein interactions in endogenous cellular extracts that retain principles of cellular organization.

As a proof-of-concept, he will present the family of gigantic oxoacid dehydrogenase complexes, which is ancient in origin and has a central role in cellular respiration. By combining biochemical, biophysical, cryo-EM and computational structural methods involving artificial intelligence, a previously uncharacterized megadalton enzymatic nanocompartment and its mechanistic implications are revealed for the first time.

This webcast will inform on the potential of native cell extracts for perceiving the architecture of cellular pathways, inspire future research on deeper analyzing the molecular origins of disease, and provide an integrated tool for understanding cellular function in a closer-to-native setting.

Learn how:

• Endogenous, transient interactions may be captured in a cell-free environment
• Heterogeneous specimens can be studied with integrative methods involving cryo-EM
• Artificial intelligence approaches can catalyse the interpretation of identified macromolecules in complex specimens