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Flow cytometry is a powerful technique for the analysis and isolation of cells based on their physical and biochemical properties. As the complexity and diversity of biological systems are increasingly revealed, there is a growing need for high-parameter cell analysis to characterize the heterogeneity and assess functionality of specific cell populations.

In recent years, spectral flow cytometry has emerged as a powerful technology that enables higher-dimensional cell analysis and sorting by capturing the full emission spectra of each fluorophore. This newer approach has pushed the boundaries for improved fluorescent dyes that can be used together without compromising biology as panels get larger and thus contributes to advancing research in various fields, such as immunology, cell biology, cancer, stem cells, and microbiology.

This webcast will review the principles and concepts of spectral flow cytometry, describe current technology innovations, and showcase some of the exciting discoveries made possible by spectral flow cytometry, while also discussing practical tips and best practices for researchers who want to adopt this technology.

• The principles of spectral flow cytometry
• The current state of spectral flow cytometry technology
• Key considerations in designing your own spectral flow cytometry experiment