Structural lipidomics using Real-Time Library Search

Or, how mass spectrometry with on-the-fly software improves phospholipid characterization

Available On Demand

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Structural lipidomics using Real-Time Library Search

Or, how mass spectrometry with on-the-fly software improves phospholipid characterization

Available On Demand

Overview

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In mass spectrometry-based lipidomics, complex lipid mixtures undergo chromatographic separation, ionization, and detection using high resolution, accurate mass (HRAM) mass spectrometry to simultaneously quantify and structurally characterize eluting species. The reported structural variety of identified lipids is strongly reliant on the analytical techniques utilized.

This webcast will present a novel lipidomics Real-Time Library Search (RTLS) approach on an Orbitrap Tribrid mass spectrometer that uses real-time lipid class identifications to trigger class-targeted MSⁿ to improve the structural characterization of glycerophospholipids and sphingomyelins in the positive ion mode. This class-based RTLS method demonstrates improved selectivity compared to current methodology, enhancing lipid characterization applications.

You will learn:

How recent technology advancements improve lipid analysis
What type of samples can be analysed using on-the-fly mass spectrometry
Tips and tricks to gaining more confident lipid annotation

This webcast has been produced by Thermo Fisher Scientific, who retains sole responsibility for content. About this content.

Presenters

Dr. Dain Brademan

Postdoctoral Fellow
Morgridge Institute for Research University of Wisconsin–Madison
View Biography

Moderator: Nikki Forrester

Freelance science
writer and editor
View Biography