Currently, the most commonly used methods for adeno-associated viral (AAV) vector quantitative analysis are qPCR and ELISA assays, along with analytical ultracentrifugation (AUC) and transmission electron microscopy (TEM). However, the time to results when using these methods can range from days to several weeks, and the acceptable tolerance range is high. Rapid, reliable in-process testing offers a significant benefit to AAV downstream process development, and can be achieved with Slope Spectroscopy utilizing Variable Pathlength Technology (VPT).

In this webinar, we will demonstrate how the CTech SoloVPE System, when used at-line in one or more stages of a process, can help to quickly identify process characteristics, provide key insights and allow for process optimization in minutes. VPT has enabled at-line measurement of concentration by eliminating the need for sample dilution or manipulation and delivering rapid and highly accurate real-time results.

• Introduction to Slope Spectroscopy – a different method for UV-Vis concentration measurement
• Review the Gene Therapy workflow for potential opportunities to accelerate testing to drive process efficiencies
• Present gene therapy case studies demonstrating the CTech SoloVPE System performance