Pushing the limits of multiplexed single-cell proteomics

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Pushing the limits of multiplexed single-cell proteomics

Available On Demand

Overview

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Single-cell proteomics has recently emerged as a promising new field capable of elucidating the vast cellular heterogeneity that defines biology, disease, and therapeutic response.

However, it is also extremely challenging, since accurate identification and quantification must be performed across entire proteomes from vanishingly small amounts of material analyzed over short, single-shot LC-MS runs. This necessitates extremes of performance from nano-flow LC and MS systems and their optimization for maximal sensitivity, speed, and depth.

This webcast will cover a roadmap for single-cell proteomics parameter optimization for human immune cell profiling using the tandem mass tag (TMT) multiplexed SCoPE-MS methodology on Thermo Scientific^™ Vanquish^™ Neo UHPLC System and Thermo Scientific^™ Orbitrap Exploris^™ 480 MS platforms. It will also describe recent advances in TMT complement ion quantification using on-the-fly phased spectrum decomposition method (SDM or phi-SDM) super-resolution signal processing via GPU-compute box architecture and complement ion data analysis within the Thermo Scientific^™ ProteomeDiscover^™ software framework.

These TMT complement ion approaches may help usher in a new era of highly accurate multiplexing in next-gen deep proteomics using wide-window or data-independent acquisition (DIA) methods.

You will learn:

The advantage of performing TMT-based single-cell proteomics
Latest MS advances to benefit single-cell proteomics
Single-cell proteomics MS parameter optimization for human immune cell profiling

This webcast has been produced by Thermo Fisher Scientific, who retains sole responsibility for content. About this content.

Presenters

Dr. David H. Perlman

Biopharma Consultant
Merck Exploratory Science Center Cambridge
View Biography

Moderator: Nikki Forrester

Freelance science
writer and editor
View Biography