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Super-resolution optical microscopies have fundamentally changed the imaging landscape, providing life scientists with a suite of new research tools. However, such techniques can be difficult to implement, necessitating different compromises that can ultimately limit their applicability. Optical pixel reassignment provides a method for super-resolution that is implemented in the context of a traditional spinning disk confocal microscope.

This webcast will include an explanation of optical pixel reassignment by Dan Collins, followed by examples of how it is being applied in cell and developmental biology research.

In one example, Dr. Laurence Pelletier will discuss use of “smart” automated standard-to-super-resolution imaging pipelines to unravel the mysteries of centrosome biogenesis and function, and how the molecular mechanisms underlying their function can go awry in disease states. In another example, Dr. Kenneth Prehoda will describe live imaging of neural stem cells using both traditional and super-resolution imaging to reveal the plasma membrane’s role in neurogenesis.

• Can super-resolution confocal microscopy capture dynamic processes in live cells?
• The difference between conventional and super-resolution spinning disk confocal microscopy systems
• Types of research questions that can be addressed using super-resolution spinning disk confocal microscopy