Understanding of DNA repair mechanisms could advance treatments for cancer and diseases of aging. But reconstituting DNA repair protein complexes from cancerous tissues to study their mechanisms of action is often time-consuming or, in some cases, impossible. A new technique performing dynamic single-molecule analysis directly on nuclear extracts allows rapid mechanistic analysis of mutant proteins from cancer cells, providing previously unseen insights into their mechanisms of action. This new innovative tool, when combined with rapid data analysis, represents a bridge between the study of biochemistry of purified proteins and molecular biology.

This webcast will describe the workflow and benefits of this new technique when combined with the LUMICKS C-Trap instrument, and will highlight a few instructive examples to demonstrate how this technique is accessible to a wide range of scientists.

• How to obtain direct evidence of mode of action of DNA repair protein complexes purified from cancer cells
• How to gain real-time, dynamic insights into DNA-protein interactions at single-molecule resolution
• Required steps to go from nuclear extract to a unique understanding of protein complex mechanisms in 2 weeks or less