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ToxTracker assay for high-throughput screening of genetic toxicity
Originally aired; June 14, 2017
With the increasing production of new chemicals for a wide range of applications in health care, food and cosmetics comes the demand for rapid and reliable approaches for toxicity assessment. Novel innovative in vitro systems should ideally not only identify toxic properties of chemicals, but also provide insight into the type of cellular damage inflicted in order to more reliably predict human health hazard of novel compounds.
ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signalling pathways upon exposure to unknown compounds (Hendriks et al, Tox Sci 2016). ToxTracker contains six different GFP-tagged reporters that allows discrimination between induction of different types of DNA damage, various cellular anti-oxidant responses and activation of the unfolded protein response. The ToxTracker reporter cell lines are combined in 96-well plates, exposed to the test samples, typically for 24h, and induction of the fluorescent reporters is examined by flow cytometry (MilliporeSigma Guava® easyCyte).
The integrative approach of the ToxTracker assay provides a powerful tool for in vitro cancer hazard screening of chemicals by unveiling activation of specific cellular signalling pathways upon exposure and deliver insight into the underlying mechanism of toxicity.
In this webcast you will learn:
- A novel approach to toxicity screening
- How the unique microcapillary fluidics design of the Guava® flow cytometers enables direct sampling, conferring the distinct advantage of absolute cell counts, and permitting direct correlation between cellular responses and cytotoxicity
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